--- title: "Get Started with glyvis" output: rmarkdown::html_vignette vignette: > %\VignetteIndexEntry{Get Started with glyvis} %\VignetteEngine{knitr::rmarkdown} %\VignetteEncoding{UTF-8} --- ```{r, include = FALSE} knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) ``` > "The simple graph has brought more information to the data analyst's mind than any other device." — John Tukey Data visualization isn't just another step in your analysis pipeline—it's where insights come alive. As visual creatures, we process charts and graphs far more intuitively than walls of numbers and text. `glyvis` brings this visual power to the `glycoverse`, offering lightning-fast and effortless visualization for your glycomics data. Built as the perfect companion to `glystats`, it transforms complex statistical results into clear, compelling visuals. ```{r setup} library(glyvis) library(glyexp) library(glyclean) library(glystats) ``` Let's dive in with some real data to see `glyvis` in action. We'll work with the `real_experiment` dataset bundled with `glyexp`— a compelling real-world N-glycoproteomics study featuring 12 patients across four distinct liver conditions: healthy controls (H), hepatitis (M), cirrhosis (Y), and hepatocellular carcinoma (C), with 3 samples representing each condition. To get our data analysis-ready, we'll use `glyclean::auto_clean()` for streamlined preprocessing. ```{r} exp <- auto_clean(real_experiment) ``` ## The Dual Nature of `glyvis` Think of `glyvis` as a versatile artist with two distinct painting styles: - **`autoplot()`** — The intelligent assistant that automatically crafts suitable plots from your `glystats` results - **`plot_xxx()`** — The precision toolkit for creating specific visualizations exactly as you envision them Let's see this in action. To create a PCA plot, we can take the direct route with `plot_pca()` on our `exp` data: ```{r} #| fig-width: 4 #| fig-height: 4 plot_pca(exp) ``` Alternatively, we can take the analytical pathway: first conducting PCA analysis with `glystats::gly_pca()`, then letting `autoplot()` work its magic on the statistical results. ```{r} #| fig-width: 4 #| fig-height: 4 pca_res <- gly_pca(exp) autoplot(pca_res) ``` While the first approach gets you there quickly, the second pathway unlocks a world of possibilities with your results. You gain access to the underlying statistical objects for advanced analyses, and can craft custom `ggplot2` masterpieces tailored for publications. The beauty of `autoplot()` lies in its versatility—it speaks fluent `glystats` across nearly every analysis type. Explore [the complete reference](https://glycoverse.github.io/glyvis/reference/index.html) to discover the full spectrum of `autoplot()` capabilities and specialized `plot_xxx()` functions. ## A Philosophy on Aesthetics Let's set expectations straight: `glyvis` isn't your publication graphics department. Creating truly stunning, publication-ready figures is an art form that demands thoughtful customization. Every compelling visualization emerges from careful consideration of countless decisions: - **Focus**: What story does your data want to tell? - **Scale**: How much visual real estate will make your message shine without overwhelming? - **Layout**: How can multiple plots dance together harmoniously? - **Palette**: Which colors will captivate while staying true to your data? - **Annotation**: What labels and text will guide your reader's eye? - **Polish**: The devil's in the details—legends, fonts, ticks, axes, grids... These creative choices flow from your intimate knowledge of the data and its scientific context. `glyvis` doesn't presume to make these artistic decisions for you. Instead, think of `glyvis` as your data exploration companion. It excels at what it was born to do: transforming `glystats` results into instant, informative visuals. It's your first glimpse into the data's soul, helping you spot patterns and generate hypotheses at the speed of thought.